Drosophila katanin is a microtubule depolymerase that regulates cortical-microtubule plus-end interactions and cell migration |
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Authors: | Zhang Dong Grode Kyle D Stewman Shannon F Diaz-Valencia Juan Daniel Liebling Emily Rath Uttama Riera Tania Currie Joshua D Buster Daniel W Asenjo Ana B Sosa Hernando J Ross Jennifer L Ma Ao Rogers Stephen L Sharp David J |
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Institution: | Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA. |
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Abstract: | Regulation of microtubule dynamics at the cell cortex is important for cell motility, morphogenesis and division. Here we show that the Drosophila katanin Dm-Kat60 functions to generate a dynamic cortical-microtubule interface in interphase cells. Dm-Kat60 concentrates at the cell cortex of S2 Drosophila cells during interphase, where it suppresses the polymerization of microtubule plus-ends, thereby preventing the formation of aberrantly dense cortical arrays. Dm-Kat60 also localizes at the leading edge of migratory D17 Drosophila cells and negatively regulates multiple parameters of their motility. Finally, in vitro, Dm-Kat60 severs and depolymerizes microtubules from their ends. On the basis of these data, we propose that Dm-Kat60 removes tubulin from microtubule lattice or microtubule ends that contact specific cortical sites to prevent stable and/or lateral attachments. The asymmetric distribution of such an activity could help generate regional variations in microtubule behaviours involved in cell migration. |
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