Effect of physical restraint on oxidative stress in mice fed a selenium and vitamin E deficient diet |
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Authors: | P K South A D Smith C A Guidry O A Levander |
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Institution: | (1) United States Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Nutrient Requirements and Functions Laboratory, 20705 Beltsville, MD |
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Abstract: | Physical restraint has been associated with increased oxidative damage to lipid, protein, and DNA. The purpose of this experiment
was to determine whether physical restraint would further exacerbate oxidative stress in mice fed a selenium (Se) and vitamin
E (VE) deficient diet. Three-week-old mice were fed a Torula yeast diet containing adequate or deficient Se and VE. Menhaden
oil was added to the deficient diet to impose an additional oxidative stress. After 4 wk feeding, half the mice in each group
were restrained for 5 d in well-ventilated conical tubes for 8 h daily. Mice fed the Se and VE deficient diets had increased
liver thiobarbituric acid-reactive substance (TBARS) levels and decreased liver glutathione peroxidase (GPX1) activity and
α-tocopherol levels. Plasma corticosterone levels were elevated in restrained mice fed the deficient diet compared to unrestrained
mice fed the adequate diet. Restraint had no effect on liver TBARS or α-tocopherol levels. Liver GPX1 activity, however, was
lower in restrained mice fed the adequate diet. In addition, liver superoxide dismutase (SOD) activity was lower in the restrained
mice fed the adequate or deficient diet. Thus, under our conditions, Se and VE deficient diet, but not restraint, increased
lipid peroxidation in mice. Restraint, however, decreased antioxidant protection in mice due to decreased activities of GPX1
and SOD enzymes. |
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Keywords: | Physical restraint oxidative stress selenium vitamin E |
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