|
|||||
|
|
Expression and secretion of outer surface protein (OSP-A) of Borrelia burgdorferi from Escherichia coli |
|
| Authors: | Yung-Fu Chang Tsai-Ling Lauderdale Woo Y Lee Sang J Shin Richard H Jacobson Max J Appel Donald H Lein |
| Institution: | Diagnostic Laboratory, Department of Microbiology, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA; James A. Baker Institute for Animal Health, Department of Microbiology, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA |
| Abstract: | Abstract Outer surface protein A (OspA) is encoded by the ospA gene from Borrelia burgdorferi . This protein induces immunity against infection in mice. The cloning and expression of OspA in Escherichia coli have been previously described, but the secretion of OspA into culture media in E. coli has not yet been reported. In this report we demonstrate that a chimeric OspA protein was secreted into culture media by E. coli when it also harbors the hemolysin secretion genes hlyBD . The OspA fusion protein was also overexpressed from a T7 promoter and purified by immobilized metal ion chromatography. This was possible because the fusion protein contains ix histidyl residues in its N-terminus. |
| Keywords: | Borrelia burgdorferi OspA Secretion Overexpression |