Cell-cell interactions influence oligosaccharide modifications on mucins and other large glycoproteins |
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| Authors: | Patricia L. Stranahan Jennifer Laroe Ronda McCombs Alex Goldsmith Iffat Rahim Mark Overland David E. Pettijohn |
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| Affiliation: | (1) University of Colorado Cancer Center, 80262 Denver, CO, USA;(2) Department of Biochemistry, 80262 Denver, CO, USA;(3) Department of Biophysics and Genetics, 80262 Denver, CO, USA;(4) University of Colorado Health Sciences Center, 80262 Denver, CO, USA;(5) Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Box B188, 80262 Denver, CO, USA;(6) Present address: Colorado State University School of Veterinary Medicine, Fort Collins, CO, USA;(7) Present address: Department of Internal Medicine, University of Colorado Health Sciences Center, 80262 Denver, CO, USA |
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| Abstract: | Intratumoral phenotypic diversity is well documented with regard to tumor associated carbohydrate antigens (TACA). The factors which control the expression of these cell-surface oligosaccharides on different cells of the same tumor are not understood. We investigated the expression of a panel of mucin associated oligosaccharides in cell lines growing at different surface densities (number of cells per cm2 of growth flask). Results show that the apparent expression of extended Lea-Lex, Lea and Lex, sialyl Lea, Tn and sialyl Tn varies with density of growth by an invasive human squamous cell lung carcinoma cell line (NU6-1), a benign variant (NE-18) and the human lung epithelial cell line BEAS-2B. The results indicate that one of the factors influencing the apparent expression of mucin-associated oligosaccharides is cell-cell interactions.Abbreviations Mab monoclonal antibody - FIT fluorescein isothiocyanate - TACA tumor associated carbohydrate antigen |
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| Keywords: | mucins oligosaccharides cell-cell interactions cancer aberrant expression |
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