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The P680 Difference Spectrum Induced by Continuous Light in Malaxis monophyllos Chloroplasts
Authors:Mao Da-zhang  Chu Zhong-xi  Xu Chun-hui  Zhao Fu-hong and Dai Yun-ling
Abstract:In chloroplasts from M. monophyllos (L.) Sw., the difference spectrum of light-induced P680 which was measured under physiological temperature and neutral pH and in the presence of potassium ferricyanide was obtained by steady-state method. In contrast under the same conditions, the P680 signal was not observed in spinach chloroplasts. Either preilumination with white light or long duration treatment with potassium ferricyanide caused marked similar changes in the difference spectrum of M. monophyllos chloroplasts, i.e. besides the bleaching bands at wavelength near 680 mm and 440 nm were the same as the control, the remarkable bleaching bands at about 660 nm and 422 nm appeared. These results were discussed briefly.
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