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Callus Induction and Plantlet Regeneration from Several Organs of Angelica sinensis (Oliv.) Diels
Authors:Zhang Shi-yu and Cheng Kuo-chang
Abstract:For the culture of organs, modified B5, KU, MS, NT, 1/2 MS media were used. Callus induction and plantlet regeneration from mots, leaves, petioles, cotyledons and hypocotyls of Angelica sinensis (Oliv.) Diels have been obtained. It was found in our experiments the following results: 1. The callus induction from the rcots has arisen rapidly and increased at a high rate and with a high differentiating frequency. 2. The phytohormones play a significant role in callus induction and differentiation. 3. The suitable combination of cytokinin (6-BA) with auxins (2, 4-D, NAA) may enhance their induction and subculture, but the effect was different on the differentiation. 4. The bud was differentiated from the embryoid and the callus.
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