| Abstract: | Both increased dry wt. of the culture cells and contents of hyoscyamine and scopolamine in the culture cells were decreased slightly (decreased about 1.2% and 2.9% respectively) when non-ionic water was used instead of redistilled water in the cell suspension culture of Anisodus acutangulus. But the non-ionic water could reduce costs largely, e.g. power cost etc. (can be reduced about 1030 yuantonne medium). Since non-ionic water can now be mass-produced easily, it suits the needs of industrial production in the future. The increased dry wt. of culture cells was a litter high and contents of two alkaloids was decreased slightly (about 5.4%) in the cell suspension culture of A. acutangulus when store confectioner’s sugar was used instead of sucrose. But the applied quantities of store confectioner’s sugar and costs could be reduce 50% and 88% respectively. The suspension cells of A. acutangulus contain rich amylase (average amylase activity was 4.37 mg reducing sugar/g, fresh wt./hour), so it could use starck as their only carbon source for the growth. |
