Abstract: | This paper described the methods of isolation, purification, and in vitro translation of alfalfa leghemoglobin mRNA. It was pointed out that the mRNA of alfalfa leghemoglobin accounts for about 1.4% of total RNA, and 24% of poly (A) +-mRNA of alfalfa nodule. This mRNA has 6, 9, 26S value populations with different molecular weights of about 0.15×106, 0.28×106, 0.48×l06 respectively. These S value mRNAs were coded for different components of leghemoglobin. |