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A fixed allele at microsatellite locus LS-39 exhibiting species-specificity for the black caviar producer Acipenser stellatus
Authors:I Jenneckens  J-N Meyer  G Hörstgen-Schwark  B May  L Debus  H Wedekind  & A Ludwig
Institution:Institute for Animal Breeding and Genetics, University of Göttingen, Göttingen, Germany;Department of Animal Science, University of California, Davis, CA, USA;University of Rostock, Department of Zoology, Rostock, Germany;Institute of Inland Fisheries, GroßGlienicke, Germany;Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany
Abstract:The sturgeon microsatellite LS-39 was amplified across 10 different species of Acipenserinae and exhibited the potential to identify the black caviar producer Acipenser stellatus on a genomic DNA level. This was because of a fixed allele of 111 bp, which was absent in the other species which were investigated. Concerning the source of sturgeon species, LS-39 is the first nuclear marker described to examine black caviar. Furthermore, new light is shed on the controversial ploidy state of sturgeons. The present authors findings at this microsatellite locus support the hypothesis that extant ~120 chromosomal species are modern diploids, whereas sturgeons with ~240 chromosomes should be considered as modern tetraploids.
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