Molecular characterization of mature pollen-specific genes encoding novel small cysteine-rich proteins in rice (Oryza sativa L.) |
| |
| Authors: | Jong-In Park Hirokazu Hakozaki Makoto Endo Yoshinobu Takada Hitoshi Ito Masanori Uchida Tomihiro Okabe Masao Watanabe |
| |
| Institution: | (1) Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan;(2) Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan;(3) Division of Natural Science, Osaka Kyoiku University, Kashiwara 582-8582, Japan;(4) The 21st Century Center of Excellence Program, Iwate University, Morioka 020-8550, Japan;(5) Present address: Fukushima Meisei High School, Fukushima 960-1192, Japan;(6) Present address: Fukushima Prefecture Agricultural College, Yabuki 969-0292, Japan;(7) Present address: Higashi Shirakawa Agricultural High School, Tanakura 963-6131, Japan |
| |
| Abstract: | In our previous cDNA microarray analysis, we identified 53 mature anther-specific genes, whose function was unknown, in rice.
We reanalyzed these genes from the viewpoint of the specific amino acid motif. Out of 53 genes, three genes, Os-26, Os-32, and Os-169 (renamed as OsSCP1, OsSCP2, and OsSCP3), encoded cysteine-rich motif (Cys-X3-Cys-X13-Cys-X3-Cys), indicating that they were novel small cysteine-rich proteins. From the search of specific elements in promoter regions,
several pollen-specific elements were found. In order to determine whether three promoters were functional in pollen or not,
the gene constructs with promoter regions fused to the β-glucuronidase gene were transformed into tobacco. Histochemical analysis
showed that these promoters were active in the mature pollen grains and pollen tubes. Furthermore, OsSCP1 and OsSCP3 formed a multigene family tandemly in the rice genome. From the results, OsSCPs might have important roles in mature pollen development and pollen tube growth. |
| |
| Keywords: | Gene family Mature pollen-specific genes Oryza sativa L Promoter analysis Small cysteine-rich protein |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
