The putrescine analogue 1-aminooxy-3-aminopropane perturbs polyamine metabolism in the phytopathogenic fungus Sclerotinia sclerotiorum |
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Authors: | Andrés?Gárriz,María?C.?Dalmasso,Fernando?L.?Pieckenstain,Oscar?A.?Ruiz author-information" > author-information__contact u-icon-before" > mailto:ruiz@intech.gov.ar" title=" ruiz@intech.gov.ar" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
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Affiliation: | (1) Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de General San Martín-Consejo Nacional de Investigaciones Científicas y Técnicas (UNSAM-CONICET), Casilla de Correo 164, 7130 Chascomús, Buenos Aires, Argentina |
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Abstract: | The effects of the putrescine analogue 1-aminooxy-3-aminopropane on fungal polyamine metabolism were evaluated using Sclerotinia sclerotiorum as an experimental model. The compound inhibited ornithine decarboxylase, spermidine synthase, and S -adenosyl-methionine decarboxylase in mycelial extracts. Addition of 1-aminooxy-3-aminopropane at 1 mM to the culture medium did not reduce mycelial growth and caused a 29% decrease in free spermidine and a two-fold increase in free spermine. When added 4.5 h before the determination of ornithine decarboxylase, 1-aminooxy-3-aminopropane reduced in vivo activity of this enzyme by 40–50%. When added 48 h before the determination, 1-aminooxy-3-aminopropane at 0.01 and 0.1 mM caused a slight increase of in vivo ornithine decarboxylase activity, while it had no effect at 1 mM. Comparison of the action of 1-aminooxy-3-aminopropane with that of other inhibitors of polyamine biosynthesis suggested that its effects on in vivo ornithine decarboxylase activity resulted from a balance between direct inhibition of enzyme activity and indirect stimulation of enzyme synthesis and/or activity mediated by the decrease in spermidine levels, which in turn was due to inhibition of spermidine synthase and S-adenosyl-methionine decarboxylase. The potential of 1-aminooxy-3-aminopropane as a tool for studies on fungal polyamine metabolism and for the control of plant diseases of fungal origin is discussed.Abbreviations AdoMetDC S-Adenosyl-methionine decarboxylase - DFMO -Difluoromethylornithine - MGBG Methylglyoxalbis-[guanyl hydrazone] - ODC Ornithine decarboxylase |
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Keywords: | S-Adenosyl-methionine decarboxylase 1-Aminooxy-3-aminopropane Ornithine decarboxylase Polyamines Sclerotinia sclerotiorum Spermidine synthase |
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