Putative sites of cytokinin action during their enhancing effect on indole alkaloid accumulation in periwinkle cell suspensions

Summary The effects of cytokinins on the different branches of the indole alkaloid pathway were investigated in Catharanthus roseus cell cultures. Addition of zeatin to a 2,4-dichlorophenoxyacetic acid-containing medium decreased tryptamine levels and increased the bioconversion of secologanin to ajmalicine. Zeatin also enhanced the geraniol-10 hydroxylase activities and modified the indole alkaloid pattern. The results are discussed in the light of previous works showing that cytokinins have a positive effect on indole alkaloid accumulation in some lines of C. roseus.Abbreviations BSTFA bis(trimethylsilyl) trifluoroacetamide - CK cytokinin - 2,4-D 2,4-dichlorophenoxyacetic acid - dw dry weight - G-10H geraniol-10 hydroxylase - NAA naphthaleneacetic acid - SE standard error - TDC tryptophan decarboxylase - Z zeatin