Chemical-enzymatic synthesis, cloning and expression of a synthetic gene coding for an env protein fragment of the human T-cell leukemia virus type 1 |
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| Authors: | A Rosenthal R Ulrich H Billwitz R Frank H Bl?cker |
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| Affiliation: | Akademie der Wissenschaften der DDR, Zentralinstitut für Molekularbiologie, Berlin-Buch. |
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| Abstract: | A synthetic gene for a 88 amino acid long env protein fragment of the human T-cell leukemia virus type 1 (HTLV1) has been assembled by ligation of 35 oligodesoxyribonucleotides, which were chemically synthesized by the phosphotriester segmental support method. After cloning into the pEX vector this HTLV1 env-protein fragment was expressed in E. coli. |
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