| 家兔钙调节蛋自-红细胞膜的制备 |
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| 引用本文： | 陈恩鸿,姚仁杰,张月芳. 家兔钙调节蛋自-红细胞膜的制备[J]. 中国生物化学与分子生物学报, 1987, 3(2): 159-163 |
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| 作者姓名： | 陈恩鸿 姚仁杰 张月芳 |
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| 作者单位： | 中国科学院上海药物研究所 上海(陈恩鸿)，北京大学生物系 北京(姚仁杰)，中国科学院上海药物研究所 上海(张月芳) |
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| 摘 要： | 本文介绍用等渗咪唑缓冲液溶血,并用低温高速或常速离心机制备出一种带钙调节蛋白(简称CaM)的红细胞膜。它具有与膜稳定结合的CaM,在钙离子存在下可以激活膜上的靶酶——Ca~(2+)+Mg~(2+)-ATP酶活性,为研究CaM功能及有关药物机制提供一种简便而理想的材料。
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| 关 键 词： | 钙调节蛋白 CaM 红细胞膜 |
| 收稿时间： | 1987-04-20 |
Preparation of CaM-erythrocyte membrane of rabbit |
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| Chen,En-Hong Yao,Ren-Jie Zhang,Yue-Fang. Preparation of CaM-erythrocyte membrane of rabbit[J]. Chinese Journal of Biochemistry and Molecular Biology, 1987, 3(2): 159-163 |
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| Authors: | Chen En-Hong Yao Ren-Jie Zhang Yue-Fang |
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| Affiliation: | (Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai;Department of Biology, Beijing university, Beijing |
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| Abstract: | Calmodulin is a low-molecular weight, high affinity calcium-binding protein which mediates many of the intercellular effects of calcium in eukaryotic cells. Calmodulin has been shown to activate or modulate intercellularly a large series of protein in a calcium-dependent manner, including the Ca2++ Mg2+-ATPase. Many drugs from the therapeutic classes mentioned inhibit calmodulin-actiated functions. The studies of calmodulin are of importance to Physiology, Biochemistry, and Pharmacology.In this Paper, We present a method to prepare enhancement of Ca2+ + Mg2+-ATP are activity on erythrocyte membrane by high(39000×g) or low (1800×g) speed centrifugation with homolysis in isosmotic imidazole buffer. |
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| Keywords: | Calmodulin CaM Erythrocyte membrane |
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