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Influence of acetate on the growth of recombinant Escherichia coli JM103 and product formation
Authors:H H Lischke  L Brandes  Xiaoan Wu  K Schügerl
Institution:(1) Institut für Technische Chemie, Universität Hannover, Callinstr. 3, D-30167 Hannover, Germany
Abstract:The influence of the acetate addeed to the M9 minimal medium and to the Luria-Bertani medium without and with glucose supplement on the growth of recombinant Escherichia coli J103 with three different types of multicopy plasmids and on the production of the fusion protein SpA :: EcoRI were investigated in shake flasks without and with induction of the gene expression by a temperature shift from 30 °C to 42 °C. At the beginning of the induction of gene expression concentrated LB-medium was added to the shake flask. Without this supplement of M9 medium no gene expression occurred.List of Symbols LB Luria Bertani cultivation medium (Table 2) - M9 cultivation medium (Table 1) - P enzym activity U ml–1] - te]TCC total cell count 106 cells ml–1] - mgr specific growth rate h-1]
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