Absence of differences in polysomal RNAs from vegetative monokaryotic and dikaryotic cells of the fungus Schizophyllum commune |
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| Affiliation: | 1. College of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu, Sichuan, 610500, PR China;2. Oil & Gas Field Applied Chemistry Key Laboratory of Sichuan Province, Southwest Petroleum University, Chengdu, Sichuan, 610500, PR China;3. State Key Laboratory of Oil & Gas Reservoir Geology and Exploitation, Southwest Petroleum University, Chengdu, Sichuan, 610500, PR China;1. Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, Canada;2. Department of Laboratory Medicine and Pathology, Division of Analytical and Environmental Toxicology, University of Alberta, Edmonton, Alberta, Canada;1. Department of Bacteriology, The University of Wisconsin-Madison, Madison, WI 53706, USA;2. School of Food Science and Biotechnology, Institute of Agricultural Science and Technology, Kyungpook National University, Daegu, South Korea;3. Department of Pharmaceutical Engineering, Woosuk University, Wanju 565-701, South Korea;4. Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Academy of Agricultural Science, RDA, Suwon 441-707, South Korea;1. Engineering Research Center for Eco-Dyeing and Finishing of Textiles, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, Zhejiang, China;2. Key Laboratory of Clean Dyeing and Finishing Technology of Zhejiang Province, Shaoxing University, Shaoxing, Zhejiang 312000, China;3. College of Textile and Garment, Hebei University of Science and Technology, Hebei 050018, China;4. Department of Nonwoven Material and Engineering, College of Textiles, Donghua University, Shanghai 201620, China |
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| Abstract: | Total polysomes, including free and membrane-bound polysomes, were isolated from the monokaryotic and dikaryotic mycelial cell types of the basidiomycete Schizophyllum commune grown under submerged conditions. Sucrose gradient centrifugation showed that these isolated polysomes had a size distribution as expected for polysomes functioning in vivo and thus contained intact mRNA. RNA preparations extracted from the polysomes were used to analyze the mRNA sets in the monokaryon and dikaryon. Saturation hybridization of single-copy DNA with a vast excess of polysomal RNA and cell-free translation of polysomal RNA in a wheat germ system followed by two-dimensional gel electrophoresis of the products did not reveal significant differences between the mRNA sets in both mycelial cell types. Moreover, it was found that the sequence complexities and coding properties of polysomal RNA and total (nuclear and cytoplasmic) RNA from S. commune are not detectably different. From these results and those obtained in previous studies it was concluded that the differentiation between monokaryotic and dikaryotic mycelial cell types as controlled by A and B incompatibility genes involves differential modification of polypeptides during or after mRNA translation. |
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