Evidence for the involvement of proteinases in the differentiation of haploid cells ofPhysarum flavicomum |
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| Affiliation: | 1. Bioengineering Department, King Fahd University of Petroleum and Minerals (KFUPM), Dhahran 31261, Saudi Arabia;2. Interdisciplinary Research Center for Hydrogen Technologies and Carbon Management, King Fahd University of Petroleum and Minerals (KFUPM), Dhahran 31261, Saudi Arabia;3. Biosystems and Machines Research Center, King Fahd University of Petroleum and Minerals (KFUPM), Dhahran 31261, Saudi Arabia;1. National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang 550025, PR China;2. School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, PR China;3. National Key Laboratory of Green Pesticide, Central China Normal University, Wuhan 430079, PR China;4. BMLT, Markham College of Commerce, Vinoba Bhave University, Hazaribagh, Jharkhand 825301, India;1. Geography Department, Humboldt-Universität zu Berlin, Unter den Linden 6, 10099 Berlin, Germany;2. Institute of Transport Research Berlin, German Aerospace Center (DLR), Rutherfordstr. 2, 12489 Berlin, Germany;1. Department of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, China;2. School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, University of Hong Kong, 21 Sassoon Road, Hong Kong SAR, China;3. Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Avenue, University Town, Nanshan, Shenzhen, 518055, China;4. Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Sun Yat-Sen University, Guangzhou, 510120, China;5. Department of Medicine, University of Hong Kong, Queen Mary Hospital, Hong Kong SAR, China |
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| Abstract: | When haploid cells ofPhysarum flavicomum reach the end of the growth phase in liquid semidefined medium they differentiate to dormant microcysts. Several compounds, effective as proteinase inhibitors in cell extracts, either blocked or delayed microcyst formation in semidefined medium. In addition, the compounds (except chloroquine) affected the conversion of swarm cells to myxamoebae.N-α-p-Tosyl-l-lysine chloromethyl ketone blocked microcyst formation and the vast majority of the cells existed as swarm cells. Chloroquine was an effective inhibitor in that the cells quickly became inactive; only about one-third of the cells converted to microcysts while the remaining cells were abnormally small. Both antipain and leupeptin inhibited normal microcyst formation and those microcysts produced were morphologically aberrant. Microcysts of normal appearance were produced in the presence of pepstatin and hemin but the process was significantly delayed. |
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