Purification and identification of a cytokinin from moss callus cells |
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| Authors: | P Beutelmann L Bauer |
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| Institution: | (1) MSU/ERDA Plant Research Laboratory, 48824 East Lansing, MI, USA;(2) Present address: Institut für Allgemeine Botanik der Universität Mainz, D-6500 Mainz, Germany |
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| Abstract: | A cytokinin was isolated from the culture medium of callus cells of the moss hybridFunaria hygrometrica (L.) Sibth xPhyscomitrium piriforme Brid. The purification procedure included ethyl-acetate extraction, silver-salt precipitation, crystallization as picrate, and ion exchange chromatography. The structure of the cytokinin was confirmed as N6–( 2-isopentenyl)adenine by means of gas chromatography and mass spectrometry. The concentration of the compound in the culture medium was determined at ca. 10-6 M.Abbreviation 2iP
N6–(2-isopentenyl) adenine |
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| Keywords: | Cytokinin Funaria x Physcomitrium Moss Callus |
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