Investigating the effect of VEGF glycosylation on glycosaminoglycan binding and protein unfolding |
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Authors: | Brandner Barbara Kurkela Riitta Vihko Pirkko Kungl Andreas J |
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Institution: | Institute of Pharmaceutical Sciences, University of Graz, A-8010 Graz, Austria. |
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Abstract: | VEGF165 binding to endothelial cells is potentiated by glycosaminoglycans (GAGs). Here, we have investigated the impact of VEGF165 N-glycosylation on GAG binding. Although glycosylated VEGF165 bound to heparin with only slightly higher affinity than non-glycosylated VEGF165, the natural ligand heparan sulfate induced a conformational change only in the glycosylated protein. Unfolding studies of the VEGF proteins indicated a stabilising effect of heparin on the growth factor structure. |
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Keywords: | Growth factor Heparin Heparan sulfate |
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