Inverted repeats in the promoter as an autoregulatory sequence for TcrX in Mycobacterium tuberculosis |
| |
Authors: | Bhattacharya Monolekha Das Amit Kumar |
| |
Institution: | Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India |
| |
Abstract: | TcrY, a histidine kinase, and TcrX, a response regulator, constitute a two-component system in Mycobacterium tuberculosis. tcrX, which is expressed during iron scarcity, is instrumental in the survival of iron-dependent M. tuberculosis. However, the regulator of tcrX/Y has not been fully characterized. Crosslinking studies of TcrX reveal that it can form oligomers in vitro. Electrophoretic mobility shift assays (EMSAs) show that TcrX recognizes two regions in the promoter that are comprised of inverted repeats separated by ∼30 bp. The dimeric in silico model of TcrX predicts binding to one of these inverted repeat regions. Site-directed mutagenesis and radioactive phosphorylation indicate that D54 of TcrX is phosphorylated by H256 of TcrY. However, phosphorylated and unphosphorylated TcrX bind the regulatory sequence with equal efficiency, which was shown with an EMSA using the D54A TcrX mutant. |
| |
Keywords: | Response regulator TcrX Crosslinking Protein&ndash DNA docking EMSA |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|