Direct somatic embryogenesis from leaves,cotyledons and hypocotyls of <Emphasis Type="Italic">Hippophae rhamnoides</Emphasis> |
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| Authors: | C Q Liu X L Xia W L Yin J H Zhou H R Tang |
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| Institution: | (1) College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, P.R. China;(2) Graduate School, Beijing Forestry University, Beijing, 100083, P.R. China;(3) Phytopathology Key Laboratory, Yunnan Agricultural University, Kunming, 650201 Yunnan, P.R. China;(4) College of Forest and Horticulture, Sichuan Agricultural University, Yaan, 625014 Sichuan, P.R. China |
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| Abstract: | Plant regeneration via direct somatic embryogenesis from cotyledons, hypocotyls and leaves in seabuckthorn (Hippophae rhamnoides L.) was achieved. The influences of basal media, carbon sources, plant growth regulators (PGRs) with different concentrations
and combinations on embryogenesis capacity of explants were studied. The highest frequency of somatic embryos production and
germination was obtained on Schenk and Hildebrandt medium (SH) supplemented with 1.0 mg dm−3 kinetin and 0.2, 0.5 mg dm−3 indole-3-acetic acid. Granulated sugar was the optimal carbon source. The embryo-derived plantlets with well-developed roots
and shoots were transferred successfully to the greenhouse with a maximum survival rate of 55 %. Histological observation
revealed that the somatic embryos were similar to those of zygotic embryos. |
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| Keywords: | indole-3-butyric acid in vitro morphogenesis kinetin plant regeneration seabuckthorn |
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