Cloning and heterologous expression of hematin-dependent catalase produced by Lactobacillus plantarum CNRZ 1228 |
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| Authors: | Abriouel Hikmate Herrmann Anette Stärke Joachim Yousif Nuha M K Wijaya Agus Tauscher Bernhard Holzapfel Wilhelm Franz Charles M A P |
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| Affiliation: | Institute of Biotechnology and Molecular Biology, Federal Research Centre for Nutrition, D-76131 Karlsruhe, Germany. |
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| Abstract: | Lactobacillus plantarum CNRZ 1228 exhibited heme-dependent catalase activity under environmental conditions similar to those encountered during sausage fermentation. The 1,455-bp catalase gene (katL) was cloned and encoded a protein of 484 amino acids. Expression of katL in a heterologous host showed that katL encodes a functional catalase. PCR screening of selected strains of lactic acid bacteria for katL indicated the presence of similar genes in other strains of lactobacilli. |
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