| 尼罗蓝在筛选PHB高产菌株中的应用研究 |
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| 引用本文： | 薛林贵,赵旭,景春娥,常思静.尼罗蓝在筛选PHB高产菌株中的应用研究[J].生物技术通报,2010(3). |
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| 作者姓名： | 薛林贵 赵旭 景春娥 常思静 |
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| 作者单位： | 兰州交通大学化学与生物工程学院,兰州,730070 |
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| 基金项目： | 国家自然科学基金项目，甘肃省科技支撑计划项目 |
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| 摘 要： | 建立一种简便、高效、可靠的初筛方法是PHB高产菌株筛选的关键。以芽孢杆菌(BacillusP-9)为试验材料,对各种PHB产生菌筛选方法进行了比较,最终确定尼罗蓝染色法为一种最佳方法。并且对试验程序进行了优化,确定尼罗蓝染液的最佳浓度为0.225mg/L,菌株的最佳染色时间为培养后96h。进一步证实了尼罗蓝染色法为一种值得推广的PHB产生菌初筛方法。
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| 关 键 词： | 尼罗蓝 聚β-羟基丁酸 高产菌 筛选 荧光 Poly-β-hydroxybutyrate |
Study on the Application of Nile Blue in Selecting of High Yielding PHB Strain |
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| Xue Lingui,Zhao Xu,Jing Chune,Chang Sijing.Study on the Application of Nile Blue in Selecting of High Yielding PHB Strain[J].Biotechnology Bulletin,2010(3). |
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| Authors: | Xue Lingui Zhao Xu Jing Chune Chang Sijing |
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| Institution: | Xue Lingui Zhao Xu Jing Chune Chang Sijing(College of Chemical , Biology Engineering,Lanzhoujiaotong University,Lanzhou 730070) |
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| Abstract: | It was to establish a simple,efficient and reliable screening method is a key for selecting high yielding strains of PHB.Bacillus P-9 was used as the experimental material,and Nile blue staining method was as the best way compareing of various screening methods of PHB producing strains.The procedure of testing was optimized and determined that the best Nile blue dye concentration was 0.225 mg/L,and the best culture time of strain for dye was 96 h.The Nile blue staining method has been further confirmed that... |
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| Keywords: | Nile bule High yielding strain Selecting Fluorescence |
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