Repetitive sequences: cause for variation in genome size and chromosome morphology in the genus Oryza |
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| Authors: | Sakurako Uozu Hiroshi Ikehashi Nobuko Ohmido Hisako Ohtsubo Eiichi Ohtsubo Kiichi Fukui |
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| Affiliation: | (1) Laboratory of Rice Genetic Engineering, Hokuriku National Agricultural Experiment Station, 1–2–1 Inada, Joetsu, 943–01, Japan;(2) Faculty of Agriculture, Kyoto University, Kyoto, 606-01, Japan;(3) Institute of Molecular and Cellular Biosciences, the University of Tokyo, Tokyo, 113, Japan |
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| Abstract: | Large variation in genome size as determined by the nuclear DNA content and the mitotic chromosome size among diploid rice species is revealed using flow cytometry and image analyses. Both the total chromosomal length (r_0.939) and the total chromosomal area (r_0.927) correlated well with the nuclear DNA content. Among all the species examined, Oryza australiensis (E genome) and O. brachyantha (F genome), respectively, were the largest and smallest in genome size. O. sativa (A genome) involving all the cultivated species showed the intermediate genome size between them. The distribution patterns of genome-specific repetitive DNA sequences were physically determined using fluorescence in situ hybridization (FISH). O. brachyantha had limited sites of the repetitive DNA sequences specific to the F genome. O. australiensis showed overall amplification of genome-specific DNA sequences throughout the chromosomes. The amplification of the repetitive DNA sequences causes the variation in the chromosome morphology and thus the genome size among diploid species in the genus Oryza. |
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| Keywords: | rice (Oryza) genome size flow cytometry image analyses fluorescence in situ hybridization genome-specific repetitive DNA sequences |
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