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An overview of fungal community diversity in diseased hop plantations
Authors:Phalip Vincent  Hatsch Didier  Laugel Bernadette  Jeltsch Jean-Marc
Affiliation:Laboratoire de Phytopathologie, Ecole Supérieure de Biotechnologie de Strasbourg, Université Louis Pasteur, Illkirch-Graffenstaden Cedex, France. vincent.phalip@esbs.u-strasbg.fr
Abstract:Samples were taken from several hop fields presenting various symptoms. Fifty-nine pure filamentous fungal strains were isolated and identified through genomic DNA preparations, PCR amplification of the ribosomal DNA internal transcribed spacer region and database interrogations. The most frequent genera were Alternaria (16 isolates) and Epicoccum (14 isolates). The ecosystem was shown to be very diverse, since as many as 27 species belonging to 17 genera were recovered. Furthermore, many of the isolated fungi are known to be involved in phytopathogenesis.
Keywords:Humulus lupulus    fungi isolation    ribosomal DNA    phytopathology    96-well devices
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