| 正相和反相柱层析组合分离纯化紫杉醇 |
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| 引用本文： | 张志强 苏志国. 正相和反相柱层析组合分离纯化紫杉醇[J]. 生物工程学报, 2000, 16(1): 69-73 |
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| 作者姓名： | 张志强 苏志国 |
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| 作者单位： | 1. 大连理工大学生化工程研究所,大连,116012
2. 中国科学院化工冶金研究所生化工程国家重点实验室,北京,100080 |
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| 基金项目： | 国家自然科学基金重点项目(29736180)。 |
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| 摘 要： | 采用正相氧化铝柱层析和反相C18柱层析从东北红豆杉培养细胞浸提物中分离纯化了紫杉醇。优化了氧化铝柱层析和反相柱层析的操作条件。实验发现，经过氧化铝柱层析后，测得的紫杉醇量大大增加。经两步层析，使紫杉醇的含量从小于1.0%提高到95%，样品中微量杂质继以重结晶步骤除去，即可获得纯度超过98%的紫杉醇晶体。采用13-CNMR对晶体分析，所得产物结构与文献上紫杉醇的结构一致。
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| 关 键 词： | 紫杉醇，正相柱层析，分离纯化 |
| 文章编号： | 1000-3061(2000)01-0069-05 |
| 修稿时间： | 1998-10-29 |
Separation and Purification of Taxol Using Normal-and Reversed-phase Chromatography in Tandem |
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| ZHANG Zhi-qiang,SU Zhi-guo. Separation and Purification of Taxol Using Normal-and Reversed-phase Chromatography in Tandem[J]. Chinese journal of biotechnology, 2000, 16(1): 69-73 |
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| Authors: | ZHANG Zhi-qiang SU Zhi-guo |
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| Affiliation: | ZHANG Zhi-qiang;(Biochemical Engineering Institute, Dalian University of Technology, Dalian 116012);SU Zhi-guo;(Institute of Chemical Metallurgy, The Chinese Academy of Sciences, Beijing 100080) |
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| Abstract: | A method is described for separation and purification of taxol from the extract of Tascus cuspidate callus cul-ture with Al2O3 normal-phase chromatography and C18 reversed-phase chromatography, The operating conditions of the two chromatographic steps were investigated and optimized. The content of taxol was greatly increased after Al2O3 chro-matography. The purity of taxol reached 95 % from less than 1.0 % after the two chromatographic steps. The final purity of taxol was more than 98% after the process of crystallization. The 13CNMR spectrum of the product was identical to that of taxol reported in the literature. |
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| Keywords: | Taxol normal-phase column chromatography separation and purification |
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