The Bacillus subtilis cell division protein FtsL localizes to sites of septation and interacts with DivIC |
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Authors: | Sievers J Errington J |
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Institution: | Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK. |
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Abstract: | FtsL is a small bitopic membrane protein required for vegetative cell division and sporulation in Bacillus subtilis. We investigated its localization by fluorescence microscopy using a green fluorescent protein (GFP) fusion. GFP-FtsL was localized at mid-cell in vegetative cells and at the asymmetric septum in sporulating cells. We also show that FtsL forms a ring-like structure at the division site and that it remains localized at mid-cell during the whole septation process. By yeast two-hybrid analysis and non-denaturing polyacrylamide gel electrophoresis (PAGE) with purified proteins, FtsL was found to interact with another membrane-bound division protein, the FtsL-like DivIC protein. |
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