Hlad Immobilization in Polyacrylamide Gels

The immobilization of horse liver alcohol dehydrogenase in a cross-linked acrylamide-N,N¹-methylenebisacrylamide copolymer gel is described. The influence of monomer concentration, the degree of cross-linking and the polymerization technique on enzyme entrapment is studied. A bead-polymerization process produced the most useful and stable immobilized enzyme preparations.