首页 | 本学科首页   官方微博 | 高级检索  
     


Three-color intranuclear staining for measuring mitosis and apoptosis in cells transfected with a GFP-tagged histone
Abstract:Abstract

Green fluorescent protein (GFP) has become a powerful tool for monitoring the expression of transfected genes by flow cytometry including GFP-tagged histones for tracking chromatin and elucidating histone function. We describe here a method for simultaneous detection of three nucleus-localized signals: a GFP-tagged histone, DNA content and detection of phosphorylated histone H3, which labels mitotic cells. We also demonstrate another application of this method for simultaneous detection of a GFP-tagged histone, DNA content, and cleaved caspase-3.
Keywords:apoptosis  flow cytometry  green fluorescent protein  histone  mitosis
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号