Abstract: | AbstractResting cells of Gordonia terrae with nitrilase (EC 3.5.5.1) activity were investigated for transformation of p-hydroxybenzonitrile to p-hydroxybenzoic acid. The maximum conversion was observed in 0.1 M potassium phosphate buffer, pH 8.0, using 40 mM substrate and resting cells corresponding to 0.70 Uml? 1 nitrilase activity at 35°C. A 500 mL fed batch reaction was designed for synthesis of p-hydroxybenzoic acid with six feedings of substrate at an interval of 1 h. A total of 14.4 g of p-hydroxybenzoic acid (> 98.7%) was obtained in 6 h with a productivity of 0.78 gh? 1g? 1DCW of G. terrae. |