Localization of Myosin,Actin, α-Actinin,Tropomyosin and Vinculin in Surface-Activated,Spreading Human Platelets |
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Abstract: | We observed the localization of the contractile proteins myosin, filamentous actin, α-actinin, tropomyosin, and vinculin in surface-activated, spreading human platelets using a single fluorescence staining procedure and conventional fluorescence microscopy. Myosin was distributed in a speckled pattern that extended radially from the granulomere. F-actin demonstrated cable-networks. Tropomyosin and α-actinin occurred in a punctuate distribution, and vinculin was localized at adhesion sites. Although myosin, F-actin, α-actinin, tropomyosin, and vinculin were not studied in resting platelets, our data support the idea that these contractile proteins are reorganized and reassembled in activated platelets during platelet function. |
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Keywords: | actin α-actinin human platelets myosin tropomyosin vinculin |
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