Uterine growth responses of the mature castrate rat to estradiol-17B |
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Authors: | L A Lavia C Shideler N Farley N Walker W Fields D K Roberts |
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Institution: | 1. Department of Obstetrics and Gynecology, University of Kansas School of Medicine — Wichita, Wichita, Kansas 67214 USA;2. Department of Pathology, University of Kansas School of Medicine — Wichita Wichita, Kansas 67214 USA;3. Department of Obstetrics and Gynecology, Wesley Medical Center Wichita, Kansas 67214 USA;4. Department of Laboratory Medicine, Wesley Medical Center Wichita, Kansas 67214 USA |
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Abstract: | To examine estrogen-stimulated uterine growth we have monitored changes in uterine DNA synthesis, ornithine decarboxylase (ODC) activity and protein content as well as luminal epithelial (LE) cell mitotic index and ultrastructural changes. We have utilized this model to examine castrate mature rat uterine growth as a function of time between 18 and 40 hours following a single injection of 25.0 ug of estradiol-17B. LE cell mitotic index and protein content increases were maximally elevated as early as 18 hours postinjection while uterine ODC activity was maximal at 28 hours; uterine DNA synthesis increases continued throughout the experiment. In addition, the infusion of either 1 or 2 ug E2 plus progesterone over a 24 hour period, stimulated elevated ODC activity under both treatment regimens and LE cell mitotic index which was inversely related to E2 dose. |
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Keywords: | To whom reprint requests should be sent |
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