Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata |
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| Authors: | Jamsari Amirul Firdaus Jamaluddin Min-Pau Tan Siti-Azizah Mohd Nor |
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| Institution: | School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia. |
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| Abstract: | Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata. |
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| Keywords: | Channa striata population genetics microsatellite multiplex PCR |
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