Cholesterol-dependent tetanolysin damage to liposomes |
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Authors: | Carl R. Alving William H. Habig Katharine A. Urban M. Carolyn Hardegree |
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Affiliation: | 1. Department of Immunology, Walter Reed Army Institute of Research, Washington, DC 20012 U.S.A.;2. Division of Bacterial Products, Bureau of Biologics, Food and Drug Administration, Bethesda, MD 20014 U.S.A. |
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Abstract: | Tetanolysin caused membrane damage, resulting in release of trapped glucose from liposomes containing cholesterol. Maximum glucose release occurred from liposomes that contained 50 mol% cholesterol. At higher or lower levels of cholesterol, glucose release was reduced and glucose release did not occur at all below 40 mol% cholesterol. The apparent activity of tetanolysin was not influenced by temperature (24°C compared to 32°C) or by liposomal phospholipid fatty acyl chain length. We conclude that tetanolysin caused cholesterol-dependent lysin-mediated damage to liposomes, possibly by means of a pore consisting of a complex of toxin and cholesterol. |
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Keywords: | Tetanolysin Liposome Cholesterol (Clostridium tetani) |
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