| Abstract: | The circular dichroism of amphotericin B “Fungizone” has been used to study its interaction with lecithin, dimyristoyl and dipalmitoyl phosphadidylcholine vesicles with or without cholesterol. It appears that circular dichroism monitors species other than those monitored by electronic absorption. As a result of association, new spectra appear which are of opposite signs according to wether the vesicles are in the gel state or in the liquid crystalline state; the cholesterol dependance of the rate of these changes is also different according to the state of the vesicles. It is concluded that the model proposed by Hsu Chen and Feingold for the gel state should not be rejected on the basis of data obtained in the liquid crystalline state. |
