Interactions of La3+ with phosphatidylserine vesicles binding,phase transition,leakage and fusion |
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| Authors: | M.M. Hammoudah S. Nir T. Isac R. Kornhauser T.P. Stewart S.W. Hui W.L.C. Vaz |
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| Affiliation: | 1. Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, NY 14263 U.S.A.;2. Department of Biophysics, Roswell Park Memorial Institute, Buffalo, NY 14263 U.S.A.;3. Max Planck Institute, Göttingen F.R.G. |
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| Abstract: | The interaction of La3+ with phosphatidylserine vesicles is elucidated by binding studies, differential scanning calorimetry, X-ray diffraction, freeze fracture electron microscopy, and release of vesicle contents. La3+ effectively competes with Ca2+ for phosphatidylserine binding sites. The saturation level is close to a La/lipid ratio of 1:3. A concentration of 0.1 mM of La3+ is sufficient to induce fusion between sonicated vesicles. |
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| Keywords: | Membrane fusion Phase transition (X-ray diffraction, Electron microscopy, Differential scanning calorimetry) |
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