The effect of diamide on amino acid transport by rat renal cortex slices |
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Authors: | Robert Reynolds Claire Rea Pamela D McNamara Stanton Segal |
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Institution: | The Division of Biochemical Development and Molecular Diseases, Children''s Hospital of Philadelphia, and the Departments of Pediatrics and Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 U.S.A. |
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Abstract: | Diamide directly added to renal cortical slices inhibits the uptake of amino acids. Steady-state kinetic analysis indicates an inhibition of α-amino acid influx without effect on efflux. The effect could be reversed by addition of pyruvate to the incubation medium. Although there was a good correlation of the transport effect of diamide with its ability to decrease cellular reduced glutathione concentration, there did not appear to be a necessary connection between them. This was shown by the fact that renal cortical slices stored at 4°C have no alteration in amino acid uptake despite the fact that GSH concentration is as low as that seen with diamide. Diamide was shown to have a direct effect on the uptake of glycine by isolated renal brush border membrane vesicles. |
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Keywords: | Amino acid transport Diamide inhibition Glutathione (Renal cortex) |
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