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Electrophysiology of phagocytic membranes II. Membrane potential and induction of slow hyperpolarizations in activated macrophages |
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| Authors: | George A Dos Reis Pedro Muanis Persechini JoséMarcos C Ribeiro Gilberto M Oliveira-Castro |
| Institution: | Instituto de Biofisica, Universidade Federal do Rio de Janeiro, Rio de Janeiro Brazil |
| Abstract: | The potential differences measured on the cell surface and after penetration into the cytoplasm of activated macrophages are described. Linear regressions are made of the measured potential differences as functions of the tip potential of each microelectrode. The surface potential of the macrophage is not significantly different from zero.Mouse macrophages have a transmembrane potential of ?26 mV, whereas in guinea-pig cells this value is ?18 mV. The input resistances of guinea-pig cells are higher than those of mouse macrophages. The cytoplasmic location of the electrode was characterized both by fluorescent dye injection and by electric criteria.Slow membrane hyperpolarizations are directly elicited by mechanical stimulation. Electric responses evoked by current pulses were further characterized.Our results lead to the establishment of objective criteria to validate intracellular recordings from macrophage. |
| Keywords: | Membrane potential Electrophysiology Electrode potential (Macrophage membrane) HEPES To whom correspondence should be addressed at: Instituto de Biofisica da UFRJ C Ciencias da Saúde Ilha do Fundão Rio de Janeiro RJ CEP 21 941 Brazil |
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