Lipid distribution in Acholeplasma laidlawii membrane. A study using the lactoperoxidase-mediated iodination |
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Authors: | Z Gross S Rottem |
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Institution: | Biomembrane Research Laboratory, Department of Clinical Microbiology, The Hebrew University-Hadassah Medical School, Jerusalem Israel |
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Abstract: | The lactoperoxidase-mediated radioiodination has been applied to study the transbilayer distribution of phospho- and glycolipids in Acholeplasma laidlawii membranes. After radioiodination, about 5% of the 125I-iodine was found in membrane lipids. A comparison of the labeling intensities of the various lipid species between iodinated intact cells and isolated membranes revealed that the glycolipids monoglucosyldiglyceride and diglucosyldiglyceride are located almost exclusively in the outer half of the bilayer, whereas the phospholipids phosphatidylglycerol and diphosphatidylglycerol as well as the phosphoglycolipids glycerophosphoryl-diglucosyldiglyceride and glycerophosphoryl-monoglucosyldiglyceride are almost equally distributed in the outer and inner halves of A. laidlawii membranes. |
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Keywords: | Lipid distribution Lactoperoxidase Iodination (Acholeplasma laidlawii membrane) |
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