Disaturated and dipolyunsaturated phospholipids in the bovine retinal rod outer segment disk membrane |
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Authors: | George P. Miljanich Larry A. Sklar Drina L. White Edward A. Dratz |
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Affiliation: | Division of Natural Sciences, University of California, Santa Cruz, CA 95064 U.S.A. |
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Abstract: | Thin-layer chromatography was used to separate the major phospholipid headgroup classes of the rod outer segment disk membrane into subfractions which differ markedly in fatty acid composition. At least 18% of the rod outer segment phosphatidylcholine must contain two saturated fatty acids. Furthermore, two unsaturated fatty acids are found in at least 43% of the phosphatidylserine, 24% of the phosphatidylcholine, and 24% of the phosphatidylethanolamine. The unsaturated acids are predominantly polyunsaturated in all cases. A similar separation, but with less resolution, was achieved with silicic acid column chromatography.The temperature dependence of the polarization of the fluorescence of trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid) showed that the thermal behavior of aqueous dispersions of the phosphatidylcholine subfractions was consistent with their fatty acid compositions. |
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Keywords: | Phospholipid Acyl chain distribution Rhodopsin (Rod outer segment membrane) |
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