A rapid method for enumerating Salmonella in milk powders |
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| Authors: | G.H. Fleet T. Karalis A. Hawa T. Lukondeh |
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| Affiliation: | Department of Food Science and Technology, University of New South Wales, PO Box 1, Kensington, New South Wales, 2033, Australia |
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| Abstract: | A one-day procedure for enumerating Salmonella in milk powder is described. Milk powder suspension was treated with a mixture of trypsin and Tween 80, then centrifuged to give a sediment of microbial cells that was plated on the xylose lysine desoxycholate agar for the direct enumeration of Salmonella. Known populations (1–200 cfu/25 g of sample) of salmonellas inoculated into milk powder suspensions were recovered with almost 100% efficiency in each of 152 trials of the method, covering 17 different serotypes. |
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