| 黑曲霉果胶裂解酶基因毕赤酵母在Pichia pastoris GS115中的表达 |
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| 引用本文： | 强慧妮,杨欣伟,田宝玉,柯崇榕,林伟铃,吕睿瑞,黄薇,王春香,黄建忠. 黑曲霉果胶裂解酶基因毕赤酵母在Pichia pastoris GS115中的表达[J]. 生物工程学报, 2009, 25(12): 1962-1968 |
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| 作者姓名： | 强慧妮 杨欣伟 田宝玉 柯崇榕 林伟铃 吕睿瑞 黄薇 王春香 黄建忠 |
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| 作者单位： | 福建师范大学,工业微生物教育部工程研究中心,生命科学学院,福建省现代发酵技术工程研究中心,福州,350108 |
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| 基金项目： | 国家自然科学基金项目(No.30800735);;福建省自然科学基金项目(No.2008F3036)资助~~ |
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| 摘 要： | 利用RT-PCR技术从黑曲霉(EIM-6)中扩增得到去除信号肽的果胶裂解酶基因A,将其插入到毕赤酵母表达载体pPIC9k上,构建重组表达质粒pPIC9K-pelA,电击转化毕赤酵母GS115,得到了表达成功的工程菌株。用终浓度为1.5%的甲醇对其进行诱导,将发酵上清液浓缩后,用盐酸法测定其酶活可以达到2.3U/mL。通过对重组毕赤酵母诱导表达产物进行SDS-PAGE鉴定,发现重组毕赤酵母分泌了1个约38kD的蛋白,与该酶基因产物的理论值相符,并通过水解圈法测定验证,均说明果胶裂解酶得到正确的分泌表达.
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| 关 键 词： | 黑曲霉 果胶裂解酶 毕赤酵母 表达 |
| 收稿时间： | 2009-09-23 |
Expression of a pectin lyase A gene from Aspergillus niger in Pichia pastoris GS115 |
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| Huini Qiang,Xinwei Yang,Baoyu Tian,Chongrong Ke,Weiling Lin,Ruirui Lü,Wei Huang,Chunxiang Wang,Jianzhong Huang. Expression of a pectin lyase A gene from Aspergillus niger in Pichia pastoris GS115[J]. Chinese journal of biotechnology, 2009, 25(12): 1962-1968 |
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| Authors: | Huini Qiang Xinwei Yang Baoyu Tian Chongrong Ke Weiling Lin Ruirui Lü Wei Huang Chunxiang Wang Jianzhong Huang |
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| Affiliation: | Engineering Research Center of Industrial Microbiology, Ministry of Education, Engineering Research Center of Fujian Modern Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou 350108, China;Engineering Research Center of Industrial Microbiology, Ministry of Education, Engineering Research Center of Fujian Modern Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou 350108, China;Engineering Research Center of Industrial Microbiology, Ministry of Education, Engineering Research Center of Fujian Modern Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou 350108, China;Engineering Research Center of Industrial Microbiology, Ministry of Education, Engineering Research Center of Fujian Modern Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou 350108, China;Engineering Research Center of Industrial Microbiology, Ministry of Education, Engineering Research Center of Fujian Modern Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou 350108, China;Engineering Research Center of Industrial Microbiology, Ministry of Education, Engineering Research Center of Fujian Modern Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou 350108, China;Engineering Research Center of Industrial Microbiology, Ministry of Education, Engineering Research Center of Fujian Modern Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou 350108, China;Engineering Research Center of Industrial Microbiology, Ministry of Education, Engineering Research Center of Fujian Modern Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou 350108, China;Engineering Research Center of Industrial Microbiology, Ministry of Education, Engineering Research Center of Fujian Modern Fermentation Technology, College of Life Sciences, Fujian Normal University, Fuzhou 350108, China |
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| Abstract: | |
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| Keywords: | Aspergillus niger pectin lyase Pichia pastoris GS115 expression |
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