Desulfurization of 2,4,6,8-tetraethyl dibenzothiophene by recombinant Mycobacterium sp. strain MR65 |
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Authors: | Kimiko Watanabe Ken-ichi Noda Jin Konishi Kenji Maruhashi |
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Affiliation: | (1) Bio-Refining Process Laboratory, Technical Cooperation Department, Japan Cooperation Center, Petroleum, 58F Sun-shine 60, 3-1-1 Ikebukuro, Toshima-ku, Tokyo, 170-6058, Japan |
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Abstract: | Recombinant Mycobacterium sp. strain MR65 harboring dszABCD genes was used to desulfurize alkyl dibenzothiophenes (Cx-DBTs) in n-hexadecane. The specific desulfurization activity for 2,4,6,8-tetraethyl DBT (C8-DBT) by DszC enzyme was about twice that for 4,6-dipropyl DBT (C6-DBT). However, the degradation rate of 2,4,6,8-tetraethyl DBT in n-hexadecane by resting cells of strain MR65 was only about 40% of that of 4,6-dipropyl DBT. These results indicated that the desulfurization ability for Cx-DBTs by resting cells depends on carbon number substituted at positions 4 and 6 and that the rate-limiting step in the desulfurization reaction of highly alkylated Cx-DBTs is the transfer process from the oil phase into the cell. |
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Keywords: | desulfurization dsz genes Mycobacterium recombinant 2,4,6,8-tetraethyl dibenzothiophene |
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