猪肺血管紧张素转换酶的提纯

本文报道了猪肺血管紧张素转换酶(ACE)的提纯方法及其鉴定,并讨论了方法的改进。肺匀浆经1.6—2.6mol/L硫酸铵沉淀,Sephadex G-200凝胶过滤,DEAE-Sephaeel及羟基磷灰石柱层析步骤,从168克肺中获得4.5毫克酶蛋白纯品。活力回收45.2％,比活力15.6单位/毫克蛋白;和匀浆上清比较,提纯390倍。经聚丙烯酰胺凝胶电泳(pH8.3)鉴定为一条带。按SDS聚丙烯酰胺凝胶电泳(SDS-PAGE)测得其分子量为132,000道尔顿。酶蛋白在-30℃貯存10月,比活力丢失30％。

PURIFICATION OF ANGIOTENSIN CONVERTING ENZYME FROM HOG LUNG

In this paper, the purification and identification of angiotensin converting enyme (ACE) from hog lung were reported and the improvements of the method were also discussed. 4.5 mg of enzyme protein from 168 gm of homogenized hog lung was obtained after purifying the latter with 1.6-2.6 mol/L ammonium sulfate fractionation, gel filtration on Sephadex G-200 column, and ion-exchange chro-matography on DEAE-Sephacel and hydroxylapatite columns. The recovery of activity was 45.2% with specific activity of 15.6 units/nig protein. ACE was purified 390-fold in comparison with the original homogenate supernatant of hog lungs.The final enzyme preparation showed one protein band on polyacrylamide gel electro-phoresis. Its molecular weight is 132,000 dalton by sodium dodecylsulfate (SDS) polyacrylamide gel method.About 30% of its specific activities is lost after storage at -30℃ for ten months.