Growth of Tetrahymena in Carbohydate-Free High-Glutamate Media |
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| Authors: | DUDLEY COX OSCAR FRANK S H HUTNER HERMAN BAKER |
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| Institution: | Haskins Laboratories, 305 E. 43rd St., New York, N.Y. 10017;Dept. of Medicine, New Jersey College of Medicine and Dentistry, Veterans Administration Hospital, East Orange, N.J. 07018;Dept. of Biology, Pace College, 41 Park Row, N.Y., N.Y. 10038;and Dept. of Biology, New York University, University Heights, Bronx, N.Y. 10453 |
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| Abstract: | SYNOPSIS. In chemically defined media, pH 6.4–6.8, supplementation with glycerol permitted growth equal to that with a carbohydrate supplement. In a pH 5.4 medium described in detail, containing L-glutamic acid 0.25%, supplementation with L-serine (essential for this strain), especially when further supplemented with proline, likewise permitted growth nearly equal to that with carbohydrate. These results are discussed in relation to the question of which components of peptone act as substrates and the resemblances of the Tetrahymena pattern of substrate utilization to that of metazoa. |
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