Protoplasten aus Zellkulturen vonDaucus carota |
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Authors: | Dipl-Biol S Hellmann Prof Dr J Reinert |
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Institution: | (1) Present address: Pflanzenphysiologisches Institut der Freien Universität Berlin, Deutschland |
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Abstract: | Summary Treatment of long-term tissue cultures of carrots and of adventive embryos in these cultures with Cellulase Onozuka P 1500 and with this enzyme + Macerocym resulted in high yields of protoplasts which could be cultivated over prolonged periods (up to 5 months) in a synthetic medium + 0.6 m sorbitol. During cultivation the size of the protoplasts increased considerably ( up to 10 times) and a high percentage of multinucleate (between 2 and 13 nuclei) protoplasts were formed. However no fusion and no regeneration of cell walls were observed. |
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