High frequency gene conversion among benomyl resistant transformants in the entomopathogenic fungus Metarhizium anisopliae |
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| Authors: | Maurí cio Reis Bogo,Marilene Henning Vainstein,francisco José Lima Aragã o,Elí bio Rech,Augusto Schrank |
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| Affiliation: | Centro de Biotecnologia, Departamento de Biotecnologia, Universidade Federal do Rio Grande do Sul P.O. Box 15005, 90540-000 Porto Alegre, RS, Brazil; Departamento de Biologia Celular, Universidade de Brasilia, 70910-900 Brasilia, DF, Brazil; Cenargen/Embrapa, 70849-970 Brasilia, DF, Brazil |
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| Abstract: | Abstract Three different methods, (i) PEG, (ii) electroporation and (iii) biolistic, were employed to transform Metarhizium anisopliae using benomyl resistance as a selectable marker. Transformation frequencies and mitotic stability varied for each method, from 0.8 to 6.9 transformants μg−1 of DNA and 46%, respectively, by the PEG method; 1.3 to 1.8 transformants μg−1 of DNA and 67% by electroporation; and 32 to 201 transformants μg−1 of DNA and 90% by biolistic. We demonstrate by PCR that 60% of the transformants were generated by gene conversion. |
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| Keywords: | Metarhizium anisopliae Fungal transformation Electroporation Biolistic Gene conversion Benomyl resistance |
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