Performance evaluation and phylogenetic characterization of anaerobic fluidized bed reactors using ground tire and pet as support materials for biohydrogen production |
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Authors: | Barros Aruana Rocha Adorno Maria Angela Tallarico Sakamoto Isabel Kimiko Maintinguer Sandra Imaculada Varesche Maria Bernadete Amâncio Silva Edson Luiz |
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Affiliation: | a Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luis km 235, CEP 13565-905 São Carlos, SP, Brazil b Department of Hydraulic and Sanitation, University of São Paulo, Av. Trabalhador, Sãocarlense, 400-Centro, CEP 13566-590 São Carlos, SP, Brazil |
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Abstract: | This study evaluated two different support materials (ground tire and polyethylene terephthalate [PET]) for biohydrogen production in an anaerobic fluidized bed reactor (AFBR) treating synthetic wastewater containing glucose (4000 mg L−1). The AFBR, which contained either ground tire (R1) or PET (R2) as support materials, were inoculated with thermally pretreated anaerobic sludge and operated at a temperature of 30 °C. The AFBR were operated with a range of hydraulic retention times (HRT) between 1 and 8 h. The reactor R1 operating with a HRT of 2 h showed better performance than reactor R2, reaching a maximum hydrogen yield of 2.25 mol H2 mol−1 glucose with 1.3 mg of biomass (as the total volatile solids) attached to each gram of ground tire. Subsequent 16S rRNA gene sequencing and phylogenetic analysis of particle samples revealed that reactor R1 favored the presence of hydrogen-producing bacteria such as Clostridium, Bacillus, and Enterobacter. |
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Keywords: | Biohydrogen production Anaerobic fluidized bed reactor Ground tire PET 16S rRNA |
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