Multi-locus variable number tandem repeat analysis for Escherichia coli causing extraintestinal infections |
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Authors: | Amee R. Manges, Patricia A. Tellis, Caroline Vincent, Kimberley Lifeso, Genevi ve Geneau, Richard J. Reid-Smith,Patrick Boerlin |
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Affiliation: | Amee R. Manges, Patricia A. Tellis, Caroline Vincent, Kimberley Lifeso, Geneviève Geneau, Richard J. Reid-Smith,Patrick Boerlin, |
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Abstract: | Discriminatory genotyping methods for the analysis of Escherichia coli other than O157:H7 are necessary for public health-related activities. A new multi-locus variable number tandem repeat analysis protocol is presented; this method achieves an index of discrimination of 99.5% and is reproducible and valid when tested on a collection of 836 diverse E. coli. |
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Keywords: | Escherichia coli Molecular epidemiology Multi-locus variable number tandem repeat analysis (MLVA) Variable number tandem repeat (VNTR) |
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