An efficient <Emphasis Type="Italic">in vitro</Emphasis> method for mass propagation of <Emphasis Type="Italic">Tylophora indica</Emphasis> |
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Authors: | M?Faisal Email author" target="_blank">M?AnisEmail author |
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Institution: | (1) Plant Tissue Culture Laboratory, Department of Botany, Aligarh Muslim University, 202 002 Aligarh, India |
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Abstract: | A protocol of high frequency shoot organogenesis and plant establishment from stem derived callus has been developed for Tylophora indica (Burm. f.) Merrill. - an endangered medicinal plant. Callus was developed on Murashige and Skoog (MS) medium supplemented with 10 M 2,4,5-trichlorophenoxy acetic acid (2,4,5-T). Multiple shoot induction was achieved from the surface of the callus after transferring onto shoot induction medium. The highest rate (80 %) of shoot multiplication was achieved on MS medium containing 5.0 M kinetin. The developed shoots rooted best on half-strength MS medium supplemented with 0.5 M indole-3-butyric acid (IBA). The in vitro raised plantlets with well developed shoot and roots were acclimatized successfully and grown in greenhouse. |
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Keywords: | callus culture growth regulators medicinal plant shoot multiplication 2 4 5-trichlorophenoxy acetic acid |
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